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URL: http://www.addgene.org/140203
Proper Citation: RRID:Addgene_140203
Insert Name: crRNA array elcA
Organism: Parastagonospora nodorum elcA spacers, Lachnospiraceae bacterium Cas12a crRNA scaffold
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32526136
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14581; Vector Backbone:New backbone modified from pYFAC-pyroA; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: To be contransformed with pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI in strains expressing dLbCas12a-VPR or dLbCas12a(D156R). These proof-of-concept vectors are intended to work as a positive control and aid the implementation of the CRISPRa vector set, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy.
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No rating or validation information has been found for pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC.
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Source: Addgene
