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URL: http://www.addgene.org/140270
Proper Citation: RRID:Addgene_140270
Insert Name: Csrp2
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-4; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip. The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high. Homology arms subcloned by recombineering from a clone of RPCI-24 C57BL/6J mouse genomic DNA library. Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation. Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive T-A regions. Depositor confirms these bases do not affect plasmid function.
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No rating or validation information has been found for pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA.
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Source: Addgene
