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URL: http://www.addgene.org/14422
Proper Citation: RRID:Addgene_14422
Insert Name: modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15870264
Vector Backbone Description: Backbone Marker:Rene Maehr; Backbone Size:3000; Vector Backbone:pBS-cas2; Vector Types:Mammalian Expression, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: inducible shRNA expression vector. shRNA coding region can be replaced as follows: design ~80 bp oligos with 5’ BamHI site followed by mutant loxP, where the last 5 bp are replaced by the first 5 bp of shRNA sequence, followed by the remaining shRNA sequence, hairpin and as shRNA sequence, T stretch and SalI site.
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Source: Addgene
