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URL: http://www.addgene.org/14874
Proper Citation: RRID:Addgene_14874
Insert Name: DAG binding domain
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16901905
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).
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Source: Addgene
