Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/149543
Proper Citation: RRID:Addgene_149543
Insert Name: SARS-CoV-2 S (surface glycoprotein)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:860; Vector Backbone:AmpR gene, with start and stop codons; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2. Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pGBW-m4137384.
No alerts have been found for pGBW-m4137384.
Source: Addgene
