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URL: http://www.addgene.org/15182
Proper Citation: RRID:Addgene_15182
Insert Name: Gateway cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:16426460
Vector Backbone Description: Backbone Size:12000; Vector Backbone:RCAS (A); Vector Types:Retroviral, RNAi, Avian expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The RCAS-RNAi viruses have been modified so that they are now compatible with the Gateway cloning system (Invitrogen). DNA encoding the hairpin is cloned into a modified U6 vector using standard cloning techniques. The construct is then moved into the retroviral vector using Gateway reagents, thus bypassing the difficulties of cloning small fragments into RCAS. As a result, each construct can be made with a minimal number of cloning steps. See Supplemental Information of the article for a detailed protocol.
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Source: Addgene
