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URL: http://www.addgene.org/15300
Proper Citation: RRID:Addgene_15300
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:17574021
Vector Backbone Description: Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: pWZL-Neo-Myr-Flag-DEST (pWN-MF-DEST) is a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF. Created by inserting an N-terminal myr-FLAG tag and the Gateway-selectable cassette into the pWzl-Neo retroviral vector. To create specific pWN-MF-ORF vectors, perform site-specific LR recombination reactions. LR reaction: 150ng DEST vector + 50ng ENTRY vector + 10xLR Buffer + LR enzyme + dH20. Incubate at 25oC for 1 hour. Add 1uL Proteinase K (20mg/ml), and incubate at 37oC for 10min. Transform into DH5alpha cells. See Invitrogen's website for detailed protocols and for LR enzyme.
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Source: Addgene
