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URL: http://www.addgene.org/15310
Proper Citation: RRID:Addgene_15310
Insert Name: PRKACA
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:3667630
Vector Backbone Description: Backbone Marker:Dr. Richard Palmiter; Backbone Size:4100; Vector Backbone:Zem3 (pUC13 + MT prom + hGH polyA); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCalpha EV was constructed by isolating the 1318 base pair NaeI/PvuII fragment of pMCalpha and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA insert. The coding sequence can be cut out with NcoI and ApaI, which should release a 1152bp fragment.
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No rating or validation information has been found for pCalpha EV (PKA catalytic subunit Calpha).
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Source: Addgene
