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URL: http://www.addgene.org/155079
Proper Citation: RRID:Addgene_155079
Insert Name: (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
Organism: other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32249828
Vector Backbone Description: Backbone Size:7481; Vector Backbone:pLCHKO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: U6-driven hgRNA cassette encoded on anti-sense strand. The PuroR cassette contains a R246C mismatch compared to the reference sequence. The depositing lab does not believe this to be of concern but the activity of PuroR should be functionally validated prior to use.
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Source: Addgene
