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URL: http://www.addgene.org/15721
Proper Citation: RRID:Addgene_15721
Insert Name: TIF1 gamma shRNA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16751102
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pRetrosuper-GFP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Comments: shRNA oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII. Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP. Sense oligo: 5'-gatcccc GAAGATGTCT CAGAGTCTG ttcaagaga CAGACTCTGA GACATCTTC tttttggaaa-3'.
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Source: Addgene
