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URL: http://www.addgene.org/157793
Proper Citation: RRID:Addgene_157793
Insert Name: p300HAT
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31543265
Vector Backbone Description: Vector Backbone:pETduet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: ySIR2 required for expression & purification - A synthetic ORF encoding the histone deacetylase domain of S. cerevisiae protein SIR2 was amplified from a dsDNA synthesized by IDT using PCR and primers adding a 5'-proximal translation start codon and a 3'-proximal translation stop codon and XhoI restriction endonuclease recognition site. XhoI restriction endonuclease (NEB) digested PCR product encoding amino acids 87-562 of wild-type SIR2 was cloned into the pETduet-1 expression vector (Novagen) using a XhoI and a blunted NdeI restriction endonuclease digestion site (NEB).
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Source: Addgene
