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URL: http://www.addgene.org/160432
Proper Citation: RRID:Addgene_160432
Insert Name: S. cerevisiae TEF2 promoter
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33311538
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint. Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.
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Source: Addgene
