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URL: http://www.addgene.org/16133
Proper Citation: RRID:Addgene_16133
Insert Name: Ataxin1
Organism: Homo sapiens
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:16713569
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGAAATCCAACCAAGAGCGG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CTTGCCTACATTAGACCGGC-3'.
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Source: Addgene
