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URL: http://www.addgene.org/16168
Proper Citation: RRID:Addgene_16168
Insert Name: Growth factor independent 1
Organism: Homo sapiens
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:16713569
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGCCGCGCTCATTTCTCGT-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG TTTGAGCCCATGCTGCGTCT-3'. There is a P->L mutation in the second amino acid. The depositing lab is aware of this and do not believe it affects the function of the protein.
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Source: Addgene
