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URL: http://www.addgene.org/161792
Proper Citation: RRID:Addgene_161792
Insert Name: H2B-mTagBFP2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Malcolm Moore; Backbone Size:8326; Vector Backbone:pUltra-hot (Addgene Plasmid #24130); Vector Types:Mammalian Expression, Lentiviral, Multicistronic; Bacterial Resistance:Ampicillin
Comments: pUltra-hot-H2B-mTagBFP2 was cloned by Jorge L. Arias-Arias at Universidad de Costa Rica ([email protected]). This vector allows the multicistronic expression of mCherry, H2B-mTagBFP2, and one gene of interest in mammalian cells. The construct contains two 2A peptides coding sequences and two cloning positions flanked by restriction sites: Position 1 (5' XbaI – 3' BamHI): Occupied by the H2B-mTagBFP2 insert. Position 2 (5' NheI – 3' EcoRI or SaII): Available for cloning one gene of interest. The vector is suitable for transient transfection, but could also be used to produce lentiviral particles for stable expression in mammalian cells. For lentivirus packaging use the additional plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259).
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Source: Addgene
