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URL: http://www.addgene.org/16427
Proper Citation: RRID:Addgene_16427
Insert Name: Pax3
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:In Situ Template; Bacterial Resistance:Ampicillin
Comments: Modification of pBH3.2 Pax3 plasmid from Martyn Goulding, Max Plank Institute (Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J. 1991 10(5):1135-47). Deleted internal SmaI fragment using SmaI in polylinker and SmaI in insert. Antisense probes are generated by digesting with EcoRI or HindIII and transcribing with T7 RNA Polymerase.
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Source: Addgene
