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URL: http://www.addgene.org/164588
Proper Citation: RRID:Addgene_164588
Insert Name: TEV-SacB
Organism: B. subtilis
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6500; Vector Backbone:pET32a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Designed and verified by Md Rezaul Karim (PhD), the responsible scientist. This plasmid is designed to clone a target gene replacing the sacB gene using CPEC (LIC) cloning. Growth media or agar plate with sucrose works as a negative selection marker for the intact template plasmid with the sacB gene and a positive selection marker for the successfully cloned plasmid.
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No rating or validation information has been found for pET32a-Trx-His6-TEV_SacB.
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Source: Addgene
