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Plasmid Name
pSIN-TRE-H2BeGFP-rtTA2
RRID:Addgene_165494 RRID Copied  
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RRID:Addgene_165494
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Plasmid Information

URL: http://www.addgene.org/165494

Proper Citation: RRID:Addgene_165494

Insert Name: Human histone H2B

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:29944140

Vector Backbone Description: Backbone Marker:-; Backbone Size:8023; Vector Backbone:pRRL-cPPT-hPGKTMPrtTA-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin

Comments: ABOUT BACKBONE: The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162). ABOUT TEST DIGESTION: Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector).

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Data and Source Information

Source: Addgene