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URL: http://www.addgene.org/165540
Proper Citation: RRID:Addgene_165540
Insert Name: T7EI (T7 Endonuclease I)
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA Endonuclease; catalyzes the cleavage of DNA mismatches and non-beta DNA structures including Holliday junctions and cruciforms, leaving 3'-OH and 5'-phosphate. Best at C mismatches and does not recognize all DNA mismatches. To a lesser extent, cleaves across a nick in dsDNA; Key enzyme for use in genome editing mutation detection workflows. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
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Source: Addgene
