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URL: http://www.addgene.org/167680
Proper Citation: RRID:Addgene_167680
Insert Name: pEC1.2::spCas9
Organism: Streptococcus pyogenes
Bacterial Resistance: Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present. Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
