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URL: http://www.addgene.org/168924
Proper Citation: RRID:Addgene_168924
Insert Name: HCoV-NL63-Envelope
Organism: Other
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:37267226
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC). Please note that Addgene identified an IS4-like element transposase in the plasmid backbone. This should not affect Gateway cloning experiments since it is outside of the Gateway cassette.
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Source: Addgene
