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URL: http://www.addgene.org/17058
Proper Citation: RRID:Addgene_17058
Insert Name: tiggywinkle hedgehog
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7583153
Vector Backbone Description: Backbone Size:3000; Vector Backbone:T7TS; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Steve Ekker clone A, in vector pT7TS. Citation: Ekker et al. (1995), Current Biology 5:944-955. T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).
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Source: Addgene