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URL: http://www.addgene.org/17077
Proper Citation: RRID:Addgene_17077
Insert Name: Nlk1
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15151990
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
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No rating or validation information has been found for ZE36 Nlk1(C387Y)-pCS2+.
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Source: Addgene
