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URL: http://www.addgene.org/17156
Proper Citation: RRID:Addgene_17156
Insert Name: dorsalin-1
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9314535
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Plasmid History: To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc. Notes for Use: Target dorsalin-1 expression in zebrafish notochord. Citation: Du et al., 1997, J. Cell Biology.
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Source: Addgene
