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URL: http://www.addgene.org/171565
Proper Citation: RRID:Addgene_171565
Insert Name: NbVHH05-human IgG-TEV-Avi-His
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35076390
Vector Backbone Description: Backbone Marker:Ah-Ram Kim (Perrimon Lab); Vector Backbone:pMT-HGP-v3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: This is extensively engineered vector. Originally, HGP (heat shock promoter-GFP-T2A-promoter) was added into pAW vector by Gibson cloning, resulting in pAW-HGP vector. And then, the cloning part including N-term Signal peptide / FLAG peptide and C-term TEV site/Avi-tag/His-tag) was introduced into pAW-HGP vector, resulting in pAW-HGP-v2. Finally, the Actin promoter was replaced with pMT promoter, resulting in final vector pMT-HGP-v3. Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
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Source: Addgene
