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URL: http://www.addgene.org/171693
Proper Citation: RRID:Addgene_171693
Insert Name: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Organism: Mus musculus
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:33972728
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
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Source: Addgene
