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Plasmid Name
pPB_Cdc42-TomKat-sensor-CAAX
RRID:Addgene_172473 RRID Copied  
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RRID:Addgene_172473
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Plasmid Information

URL: http://www.addgene.org/172473

Proper Citation: RRID:Addgene_172473

Insert Name: tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:34785668

Vector Backbone Description: Backbone Size:6785; Vector Backbone:pPB; Vector Types:Mammalian Expression, Piggybac transposon; Bacterial Resistance:Ampicillin

Comments: This sensor is based on the design of the Matsuda lab (https://www.ncbi.nlm.nih.gov/pubmed/21976697), but modified to use tdTomato as the FRET donor and tdKatushka as the FRET acceptor. The tdKatushka2 was originally cloned by Michael Davidson's lab (Addgene plasmid #56049).

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Data and Source Information

Source: Addgene