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URL: http://www.addgene.org/173157
Proper Citation: RRID:Addgene_173157
Insert Name: sgRNA cassette under U6 promoter
Organism: Arabidopsis thaliana
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35022409
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski. Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in: Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484. https://doi.org/10.1007/978-1-0716-2253-7_13 https://pubmed.ncbi.nlm.nih.gov/35461452/
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Source: Addgene
