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Plasmid Name
ATP1A1_G4_Q118R_Dual_pegRNA
RRID:Addgene_173199 RRID Copied  
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RRID:Addgene_173199
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Plasmid Information

URL: http://www.addgene.org/173199

Proper Citation: RRID:Addgene_173199

Insert Name: ATP1A1 G4 Q118R pegRNA

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:36207338

Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin

Comments: Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.

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Data and Source Information

Source: Addgene