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URL: http://www.addgene.org/1732
Proper Citation: RRID:Addgene_1732
Insert Name: Gag-Pol and Rev
Organism: Feline Immunodeficiency Virus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:12905; Vector Backbone:modified pUC119; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
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Source: Addgene
