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Plasmid Name
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
RRID:Addgene_173211 RRID Copied  
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RRID:Addgene_173211
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Plasmid Information

URL: http://www.addgene.org/173211

Proper Citation: RRID:Addgene_173211

Insert Name: ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:36207338

Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin

Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.

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Data and Source Information

Source: Addgene