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Plasmid Name
ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA
RRID:Addgene_173213 RRID Copied  
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RRID:Addgene_173213
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Plasmid Information

URL: http://www.addgene.org/173213

Proper Citation: RRID:Addgene_173213

Insert Name: ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:36207338

Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin

Comments: Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA) to co-target ATP1A1 and RUNX1 to perform coselection for RUNX1 +1 ATG insertion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.

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Ratings and Alerts

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Data and Source Information

Source: Addgene