Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/17328
Proper Citation: RRID:Addgene_17328
Insert Name: streptavidin
Organism: Streptomyces avidinii
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7724536
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pTSA-38 is a derivative of pTSA-13 with a W120F mutation. In brief, mpSA-29 was generated by inserting a 480-bp fragment of pTSA-13 carrying the entire coding sequence into M13mpl8. Oligonucleotide-directed in vitro mutagenesis was performed on mpSA-29 by using the 30-nt oligonucleotide, 5'-ACCAGCGTGGACTTGAAG- GCGTTGGCCTCG-3', to convert the TGG codon for Trp- 120 to TTC for Phe (mpSA-36). Then, a 405-bp fragment of mpSA-36 carrying the entire coding sequence was cloned into pET-3a. The resulting expression vector, pTSA-38, encodes a streptavidin W120F mutant.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pTSA-38.
No alerts have been found for pTSA-38.
Source: Addgene
