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URL: http://www.addgene.org/17329
Proper Citation: RRID:Addgene_17329
Insert Name: streptavidin
Organism: Streptomyces avidinii
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11716692
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.
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Source: Addgene
