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URL: http://www.addgene.org/17385
Proper Citation: RRID:Addgene_17385
Insert Name: FEZ1
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15879557
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCDNA4 TO2 myc HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Rat2 FEZ1 sequences, 1179 bp, was amplified using cDNA from the wild-type Rat2 fibroblasts or the mutant R3-2 line as templates. The sequences of the PCR primers for generation of C-terminal myc-His-tagged FEZ1 were as follows: forward primer, FEZ1-S1-BamHI, 5'-GCTGATGGATCCACCATGGAGGCTC CACTGGTGAGT3'; reverse primer, FEZ1-A3-XhoI, 5'-GCAACGCTCGAGCAGGTTGGACAGAGCACTTTTAAA-3'.
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Source: Addgene
