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URL: http://www.addgene.org/175390
Proper Citation: RRID:Addgene_175390
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35361849
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mouse Targeting, Cloning vector, to be used to deliver transgene into mice with dual heterologous Bxb1 attP sites; Bacterial Resistance:Ampicillin
Comments: We recommend this plasmid for the generation of the DNA donor when the inserted transgene size is <15kb. For larger insertions, we recommend the low-copy plasmid p5155 (175391). Depending on the nature of the sequence cloned into this plasmid, it may be beneficial to grow using NEB Stable, at 30C. However, just to maintain p5154 alone, DH5alpha is adequate (though we generally use DH10Beta).
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Source: Addgene
