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URL: http://www.addgene.org/17672
Proper Citation: RRID:Addgene_17672
Insert Name: c-src
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:2582237
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: pMcsrc was constructed by gel purifying the 8.1-kilobase (kb) BamHI c-src fragment running from c-src coordinates -2.1 to 6.0 kb and ligating it into plasmid pEVX which had been linearized at a unique BglII site lying between the two Moloney murine leukemia virus (MoMLV) long terminal repeats (LTRs). The upstream BamHI site is about 270 base pairs (bp) upstream from the beginning of c-src exon no. 2, whereas the downstream site lies about 1.2 kb beyond the c-src termination codon.
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Source: Addgene