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URL: http://www.addgene.org/177410
Proper Citation: RRID:Addgene_177410
Insert Name: Bcl-XL-ActA, BclX-acta
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-XL-ActA. There is a flexible, 7 aa linker "SGLRSRE", between Venus and BclXL-ActA. This Venus protein contains an F224R mutation to make it monomeric. Note that following the stop codon at the end of the “ActA” sequence, this plasmid contains a ~1000bp insertion in the original pEGFP-C1 backbone that consists partially of duplication of the backbone itself and a sequence left over from cut and paste from another backbone. Expression of the protein of interest, Venus-BclXL-ActA, in BMK and MCF-7 cells was not affected by this insertion.
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Source: Addgene
