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URL: http://www.addgene.org/177837
Proper Citation: RRID:Addgene_177837
Insert Name: tia1l-sgRNA target site-hsp70-EGFP
Organism: Danio rerio
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:36050398
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The tia1l sgRNA was designed and published by Hwang et al., 2013, Nature Biotechnology: https://pubmed.ncbi.nlm.nih.gov/23360964/. The strategy is based on a method described by Lackner et al. 2015, Nature Communications: https://pubmed.ncbi.nlm.nih.gov/26674669/.
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Source: Addgene