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URL: http://www.addgene.org/178091
Proper Citation: RRID:Addgene_178091
Insert Name: NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36207338
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid is derived from eSpCas9(1.1) (Plasmid #71814) from Feng Zhang's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
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Source: Addgene
