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URL: http://www.addgene.org/17860
Proper Citation: RRID:Addgene_17860
Insert Name: FLIPrbs
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14550551
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.
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Source: Addgene
