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URL: http://www.addgene.org/179694
Proper Citation: RRID:Addgene_179694
Insert Name: BirA
Organism: Escherichia coli (strain K12)
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pMAL-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Express MBP-BirA fusion protein overnight at 18C. Lysis buffer: 50 mM Tris pH 8, 300 mM NaCl. Size exclusion buffer: 50 mM Tris pH 8.0, 150 mM NaCl.
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Source: Addgene
