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URL: http://www.addgene.org/182032
Proper Citation: RRID:Addgene_182032
Insert Name: Streptococcus pyogenes cas9
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:35813026
Vector Backbone Description: Backbone Size:6484; Vector Backbone:Synthetic vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: The ORF of cas9 is codon optimized for E. coli expression and was synthesized by GeneArt. The additional features (MBP, TEV sites, FLAG, 3XNLS and 12XHis tag) were either PCR amplified from various vectors or purchased as synthetic DNA fragments. All gene fragments were assembled by Gibson cloning into this customized vector.
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Source: Addgene
