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URL: http://www.addgene.org/1832
Proper Citation: RRID:Addgene_1832
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10572187
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: See scanned map. This is the original RET construct for in vitro mutagenesis experiments. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. This construct can not be used for expresssion pattern analysis because of lack of IRES-EGFP. The XbaI-XbaI fragment can be used for transfection. Enhancer sequence in the U3 portion of the 5' LTR is not included in this XbaI-XbaI fragment. (Leder #C-1021)
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Source: Addgene
