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URL: http://www.addgene.org/1837
Proper Citation: RRID:Addgene_1837
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10572187
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: See scanned map (#5). This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment. (Leder #C-1024)
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Source: Addgene
