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URL: http://www.addgene.org/184570
Proper Citation: RRID:Addgene_184570
Insert Name: LiLac fluorescent sensor of lactate
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35614105
Vector Backbone Description: Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.)
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Source: Addgene
