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URL: http://www.addgene.org/18760
Proper Citation: RRID:Addgene_18760
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:6300; Vector Backbone:MSCV IRES Luciferase; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
Comments: To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.
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Source: Addgene
