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URL: http://www.addgene.org/18786
Proper Citation: RRID:Addgene_18786
Insert Name: TRAP binding site
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17023487
Vector Backbone Description: Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).
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Source: Addgene