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URL: http://www.addgene.org/188488
Proper Citation: RRID:Addgene_188488
Insert Name: tdTomato sgRNA
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35789697
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8652; Vector Backbone:PB_rtTA_BsmBI; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid was cloned with the one step BsmBI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
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Source: Addgene