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URL: http://www.addgene.org/19302
Proper Citation: RRID:Addgene_19302
Insert Name: Ras-related GTP binding B
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18497260
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that this plasmid is actually quite toxic to cells. From the Sabatini lab:"it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells." From the paper: "To verify the actions of the Rags in a more physiological setting than that achieved by transient cDNA transfection, we generated HEK-293T cell lines stably expressing Rheb1, RagB, or RagBGTP (attempts to generate lines stably expressing RagBGDP failed)."
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Source: Addgene
